Blunt/TA Ligase Master Mix from New England Biolabs

Product Specs
ItemBlunt/TA Ligase Master Mix
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0367S
Quantity50 reactions
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemicPLoS OneAugust 25, 2023Marine Wasniewski, Franck Boué, Céline Richomme, Etienne Simon-Lorière, Sylvie Van der Werf, Flora Donati, Vincent Enouf, Yannick Blanchard, Véronique Beven, Estelle Leperchois, et al.ng DNA from each sample. Native barcode adapters NBD04 were ligated in Blunt/TA Ligase Master Mix (M0367S, NEB, USA), and the resulting product was purified using AMPure XP beads before being pooled to produce

(2) Investigations on SARS-CoV-2 and other coronaviruses in mink farms in France at the end of the first year of COVID-19 pandemicbioRxivJuly 7, 2023Marine Wasniewski, Franck Boué, Céline Richomme, Etienne Simon-Lorière, Sylvie Van der Werf, Flora Donati, Vincent Enouf, Yannick Blanchard, Véronique Beven, Estelle Leperchois, et al.ng DNA from each sample. Native barcode adapters NBD04 were ligated in Blunt/TA Ligase Master Mix (M0367S, NEB, USA), and resulting product was AMPure XP beads purified before pooling to produce a 54 μl equimass

(3) Rapid whole genome sequencing methods for RNA virusesFrontiers in MicrobiologyFebruary 23, 2023Masayasu Misu, Tomoki Yoshikawa, Satoko Sugimoto, Yuki Takamatsu, Takeshi Kurosu, Yukiteru Ouji, Masahide Yoshikawa, Masayuki Shimojima, Hideki Ebihara, Masayuki Saijoreaction volume of 25 μL containing 400 ng of cDNA, 12.5 μL of Blunt/TA ligase master mix (catalog number M0367S, New England BioLabs), 1.5 μL of Native Barcoding Expansion and H2O. The reaction mixture was incubated

(4) Nano3P-seq: transcriptome-wide analysis of gene expression and tail dynamics using end-capture nanopore cDNA sequencingNature MethodsJanuary 1, 2023Oguzhan Begik, Gregor Diensthuber, Huanle Liu, Anna Delgado-Tejedor, Cassandra Kontur, Adnan Muhammad Niazi, Eivind Valen, Antonio J. Giraldez, Jean-Denis Beaudoin, John S. Mattick, et al.was mixed with 5 µl Adapter Mix (AMX), 22.5 µl Rnase-free water and 50 µl Blunt/TA Ligase Mix (NEB, M0367S) and incubated in room temperature for 10 min. The reaction was cleaned up using 0.8× AMPure XP beads,

(5) Metagenomic sequencing for detection and identification of the boxwood blight pathogen Calonectria pseudonaviculataScientific ReportsDecember 1, 2022Shu Yang, Marcela A. Johnson, Mary Ann Hansen, Elizabeth Bush, Song Li, Boris A. Vinatzermixing 22.5 μL of the elute with the Blunt/TA Ligase Master Mix (New England Biolabs, Inc.; Catalog # M0367S) and Native Barcode (ONT; Native Barcoding Expansion Kit EXP-NBD104). Barcoded DNA was cleaned up by

(6) Systematic generation and imaging of tandem repeats reveal base-pairing properties that promote RNA aggregationCell Reports MethodsNovember 21, 2022Atagun U. Isiktas, Aziz Eshov, Suzhou Yang, Junjie U. GuoMonarch DNA Gel Extraction Kit,New England Biolabs,Cat# T1020S Blunt/TA Ligase Master Mix,New England Biolabs,M0367S Instant Sticky-end Ligase Master Mix,New England Biolabs,Cat# M0370S QIAprep Spin Miniprep Kit,Qiagen,Cat#

(7) Characterization of full-length CNBP expanded alleles in myotonic dystrophy type 2 patients by Cas9-mediated enrichment and nanopore sequencingeLifeAugust 26, 2022Massimiliano Alfano, Luca De Antoni, Federica Centofanti, Virginia Veronica Visconti, Simone Maestri, Chiara Degli Esposti, Roberto Massa, Maria Rosaria D'Apice, Giuseppe Novelli, Massimo Delledonne, et al.excision (see M&M)" Commercial assay or kit,Blunt/TA Ligase Master Mix,New England BioLabs,Cat. No.: M0367S,T4 DNA ligase for native barcode ligation to dA-tailed ends (see M&M) Commercial assay or kit,FLO-MIN106D

(8) The Chromosome-Scale Reference Genome of Macadamia tetraphylla Provides Insights Into Fatty Acid BiosynthesisFrontiers in GeneticsFebruary 23, 2022Yingfeng Niu, Guohua Li, Shubang Ni, Xiyong He, Cheng Zheng, Ziyan Liu, Lidan Gong, Guanghong Kong, Wei Li, Jin LiuUnited Kingdom) and a PCR-free “native barcoding” kit provided by ONT. Blunt/TA ligase Master Mix (M0367S, NEB) was used to ligate native barcode adapters for 10 min at room temperature. A 1:1 volume of AMPure

(9) Detection of SARS‐CoV‐2 in two cats during the second wave of the COVID‐19 pandemic in FranceVeterinary Medicine and ScienceJanuary 1, 2022Matthieu Fritz, Nicolas Nesi, Solène Denolly, Bertrand Boson, Vincent Legros, Serge G. Rosolen, Alexandra Briend‐Marchal, Meriadeg Ar Gouilh, Eric M. Leroysamples. End‐prepared DNA was ligated with native barcode adapters NBD04 using Blunt/TA Ligase Master Mix (M0367S; NEB). Following the barcode ligation reaction, the DNA was cleaned with AMPure XP beads. The two samples

(10) Genomic skimming and nanopore sequencing uncover cryptic hybridization in one of world’s most threatened primatesScientific ReportsDecember 1, 2021Joanna Malukiewicz et al.30 ul of end-prepped DNA with 20 ul SQK-LSK108 adapter Mix, and 50 ul NEB Blunt/TA Master Mix (NEB M0367S). The mix was flicked and then incubated at room temperature for 10 min. The adapter-ligated DNA was

(11) Infection with SARS‐CoV‐2 variant B.1.1.7 detected in a group of dogs and cats with suspected myocarditisVeterinary RecordNovember 4, 2021Luca Ferasin, Matthieu Fritz, Heidi Ferasin, Pierre Becquart, Sandrine Corbet, Meriadeg Ar Gouilh, Vincent Legros, Eric M. LeroyEnd‐prepared amplicons were ligated with native barcode adapters NBD04 using Blunt/TA Ligase Master Mix (M0367S, NEB, USA) and then purified with AMPure XP beads. The two samples were then pooled to produce a 60

(12) The Genome of the CTG(Ser1) Yeast Scheffersomyces stipitis Is PlasticmBioOctober 26, 2021Samuel Vega-Estévez, Andrew Armitage, Helen J. Bates, Richard J. Harrison, Alessia Buscainoa PCR-free kit with NEBNext End Repair (E6050S), NEBNext dA-tailing (E6053S), and Blunt T/A ligase (M0367S) New England Biolabs modules. Sequencing was performed on a MiSeq benchtop analyzer (Illumina) using

(13) Report of One-Year Prospective Surveillance of SARS-CoV-2 in Dogs and Cats in France with Various Exposure Risks: Confirmation of a Low Prevalence of Shedding, Detection and Complete Sequencing of an Alpha Variant in a CatVirusesSeptember 3, 2021Emilie Krafft et al.End-prepared amplicons were ligated with native barcode adapters NBD04 using Blunt/TA Ligase Master Mix (M0367S, New England Biolabs, Ipswich, MA, USA) and then purified with AMPure XP beads (1× ratio). The two samples

(14) Tissue-specific DamID protocol using nanopore sequencingJ Biol MethodsAugust 27, 2021Georgina Gómez-Saldivar, Dominique A. Glauser, Peter Meistersamples in one sequencing experiment.✓ Flow Cell Priming kit (EXP-FLP001, ONT)✓ NEB Blunt/TA Master Mix (M0367S, NEB)✓ NEBNext Quick Ligation Module (E6056S, NEB)✓ AMPure XP 1.8 beads (A63880, Agencourt)✓ Magnetic

(15) Light-Inducible Spatio-Temporal Control of TLR4 and NF-κB-Gluc Reporter in Human Pancreatic Cell LineInternational Journal of Molecular SciencesAugust 26, 2021Anna Stierschneider, Petra Grünstäudl, Katrin Colleselli, Josef Atzler, Christian T. Klein, Harald Hundsberger, Christoph Wiesnerplasmid) by blunt end ligation using Blunt/TA Ligase Master Mix (New England BioLabs, Frankfurt, Germany; M0367S) of the PCR products and transfected in Escherichia coli strain GCI-5α (GeneCopoeiaTM, 1 Rockville,

(16) Morphological and transcriptomic alterations in neonatal lamb testes following developmental exposure to low-level environmental chemical mixture.Environmental Toxicology and PharmacologyAugust 1, 2021Chris S. Elcombe, Ana Monteiro, Mohammad Ghasemzadeh-Hasankolaei, Neil P. Evans, Michelle Bellinghamusing a barcode expansion kit (Oxford Nanopore – EXP-NBD104) and Blunt/TA Ligase (New England Biolabs – M0367S). As the barcoding kit only contains twelve individual barcodes, samples were split into two groupings

(17) Comparative Analysis of Host-Associated Variation in Phytophthora cactorumFrontiers in MicrobiologyJuly 2, 2021Charlotte F. Nellist, Andrew D. Armitage, Helen J. Bates, Maria K. Sobczyk, Matteo Luberti, Laura A. Lewis, Richard J. HarrisonPCR-free method using NEBNext End Repair (E6050S), NEBNext dA-tailing (E6053S) and Blunt T/A ligase (M0367S) New England Biolabs modules. Library insert sizes were 400–600 bp and were sequenced using Illumina

(18) Direct oligonucleotide sequencing with nanoporesOpen Research EuropeMay 12, 2021Sachin Chalapati, Conor A Crosbie, Dixita Limbachiya, Nimesh Pinnamaneni100 uM concentration. Enzymes and buffers T4 PNK - NEB - M0201S Blunt/TA Ligase Master Mix - NEB - M0367S IDTE - IDT - 11-01-02-02 Page 3/8 Qubit ssDNA Assay Kit - ThermoFisher - Q10212 Nuclease Free Water

(19) Efficient Expression of Soluble Recombinant Protein Fused with Core-Streptavidin in Bacterial Strain with T7 Expression SystemMethods and protocolsDecember 1, 2020Tarar A, Alyami EM, Peng CA.USA) 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. • High fidelity Phusion polymerase (Cat No.: M0491S) Blunt/TA Ligase Master Mix (Cat No.: M0367S) T4 DNA ligase (Cat No.: M0202S) Monarch PCR & DNA cleanup kit (Cat No.: T1030S) BL21(DE3) competent

(20) Accurate Detection of HPV Integration Sites in Cervical Cancer Samples Using the Nanopore MinION Sequencer Without Error CorrectionFrontiers in GeneticsJune 26, 2020Wenjuan Yang, Ying Liu, Ruyi Dong, Jia Liu, Jidong Lang, Jialiang Yang, Weiwei Wang, Jingjing Li, Bo Meng, Geng Tianend-prepared DNA was ligated with native barcode NB04 from ONT using the NEB Blunt/TA Ligase Master Mix (M0367S; NEB), and then incubated at 25°C for 15 min. Following the barcode ligation reaction, the DNA was cleaned

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